Molecular Docking Studies on Methanolic Propolis Extracts Collected from Different Regions in Saudi Arabia as a Potential Inhibitor of Topoisomerase IIβ

Propolis is a sticky substance made by honeybees from various plant parts that rich in biologically active substances such as flavonoids, phenolic acids, and phenolics has wide range of applications the food, cosmetics, pharmaceutical industries. The current study focused on isolation honeybee propolis samples three different locations Saudi Arabia: Al Hada, Baljurashi, Rawdat Khuraim, evaluation their anti-cancer effect against human liver cancer cell lines (HeP-G2) breast (MCF-7). Five chemical compounds present methanolic extract were detected HPLC. Furthermore, molecular modeling studies conducted to explain mechanism activity exerted compounds. collected sites had MCF-7 HeP-G2. Samples Khuraim site showed highest inhibitory reaching 81.5% 83.2% HeP-G2, respectively. HPLC four main samples: pinobanksin, pinocembrin, galangin, xanthomicrol. docking technique galangin pinocembrin higher than xanthomicrol pinobanksin binding affinity with topoisomerase IIβ enzyme was much greater.